Rev.Adv.Mater.Sci.(RAMS)
No 5, Vol. 5, 2003, pages 440-449

POROUS SILICON MATRIX FOR APPLICATIONS IN BIOLOGY

Anca Angelescu1, Irina Kleps1, Miu Mihaela1, Monica Simion1, Teodora Neghina1, Stefana Petrescu2, Nicanor Moldovan3, Crina Paduraru2 and Aurelia Raducanu2

1 Institute for Microtechnologies, 32B, Erou Iancu Nicolae Street, R-72296, Bucharest, Romania,
2 Institute of Biochemistry, 296, Splaiul Independentei Street, sector 6, Bucharest, Romania
3 The Ohio State University, 310 Davis HLRI, 473 West 12th Avenue, Columbus, Ohio 43210-1252, USA

Abstract

Different porous silicon (PS) layers, with an average pore size distribution of 10 nm were realised on p+Si (111), and p+Si (100), by partial electrochemical dissolution in hydrofluoric acid based solutions. Various treatments for structure surface modification/stabilisation were made in order to ensure the PS biocompatibility: thermal treatments, carbon layers deposition; hexametildisilazane treatments, and a-SiC deposited by hexametildisilane. On these structures, human retinal endothelial cells (HREC), mouse aortic endothelial cells (MAEC), murine melanomas (B16 - F1), neuronal mouse cells (B50) and hamster ovarian cells (CHO) were grown, and studied by Laser Scanning Cytometry (LSC).

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